Getting Started

First Steps

1. Launch KymFlow: Run kymflow-gui from your terminal
2. Select a Folder: Use the folder selector to choose a directory containing kymograph TIFF files
3. Browse Files: The file table shows all TIFF files found in the selected folder
4. Select a File: Click on a file in the table to view its metadata and image

Basic Workflow

Viewing Files

• The file table displays key metadata for each file
• Select a file to see detailed metadata, image, and analysis results
• Use the image viewer to explore the kymograph data

Editing Metadata

1. Select a file from the table
2. Edit fields in the Metadata section (species, region, notes, etc.)
3. Changes are saved automatically when you save the analysis

Running Analysis

1. Select a file
2. Set the window size (typically 16, 32, 64, 128, or 256)
3. Click "Analyze Flow" in the toolbar
4. Wait for analysis to complete
5. Click "Save" to persist results

Batch Processing

1. Navigate to the Batch page
2. Select multiple files (hold Ctrl/Cmd to multi-select)
3. Set window size
4. Click "Analyze Flow" to process all selected files sequentially

Next Steps

• See GUI Usage for detailed interface documentation
• See Batch Processing for batch analysis workflows
• See API Reference for programmatic access